Initially Posted:
January 30, 2007
Revised/Updated:
February 13, 2007 |
Copyright © 2007
Chester R. Cooper, Jr. |
Laboratory Home Page for
Biology
of Fungi - BIOL 6948L
Spring 2007 Semester, Course Code 0430
|
January 30, 2007
and Februray 6, 2007
Isolation
of Fungi from a Clinical Specimen [PDF]
Resources/References:
Tape Touch Method (Harris, 2000)
[PDF]
Slide Culture Technique (Harris, 1986) [PDF]
Februray 13, 2007
Isolation
of Fungal DNA [PDF]
Resources/References:
DNA Extraction
from Filamentous Fungi (Jin et al., 2004) [PDF]
MasterPure Kit
Instructions [PDF]
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Quantification and Purifty of
Genomic DNA
Using athe SmartSpec 3000 Spectrophotometer [PDF]
Resources/References:
SmartSpec 3000
Manual [PDF]
Februray 20, 2007
Amplification
of Selected Gene Fragments
From Fungal DNA Using the Polymerase Chain Reaction (PCR) [PDF]
Resources/References:
AmpliTaq Gold Master Mix Protocol [PDF]
*************************************************************************
Analysis of PCR Products [PDF]
Resources/References:
Amresco
100-bp laddder [PDF]
*************************************************************************
Purification of PCR Products [PDF]
Resources/References:
QIAquick Spin Handbook [PDF]
Februray 20, 2007
Cloning of PCR Products [PDF]
Resources/References:
TOPO TA Cloning Manual [PDF]
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Course
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Copyright © 2007
Chester R. Cooper, Jr.
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