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© 2018 Chester R. Cooper, Jr.

BIOL 3702L Home Page      Laboratory Exercises      Safety Rules

BIOL 3702L Schedule of Laboratory Activities

Spring 2019 Semester

(updated February 17, 2019)

This web page contains links to each laboratory exercise.  Each exercise can be downloaded as a PDF document.*  Similarly, many of the exercises are accompanied by a PDF document containing the laboratory lecture notes.  There are also some links to other web pages associated with this laboratory course.  For example, links have been provided to the CONNECT learning platform for each LearnSmart Lab Exercise.  However, links to other relevant materials (e.g., videos, vendor instructions, etc.) coupled with a given exercise are not provided.  These links can be found on the Laboratory Exercises web page.

Please note that LearnSmart Lab Exercises shall be available at 8:00 AM (either a Monday or Wednesday) on the date indicated and close at 5:00 PM on the scheduled date (either a Tuesday or Thursday).  There shall be no exceptions for missing a deadline.

*Requires Adobe Acrobat Reader

Please report any “broken” links to Dr. Cooper.

Day/Date

Scheduled Activities

[Unless noted, all exercises are to be performed by each student]

Mon., Jan. 14

Tue., Jan 15

Introduction to the Microbiology Laboratory

·       Lecture Notes: Introduction to the Microbiology Laboratory

·       Review of Safety Rules

·       LearnSmart Lab Exercise: Lab Safety (open)

Wed., Jan. 16

Thu., Jan. 17

·       Exercise: The Bright-Field Microscope: Its Proper Use and Care

·       Lecture Notes: Bright-Field Microscopy

·       LearnSmart Lab Exercise: Microscope Microbiology (open)

·       LearnSmart Lab Exercise: Aseptic Technique (open)

·       LearnSmart Lab Exercise: Isolation Methods (open)

Mon., Jan. 21

Laboratory Closed – Martin Luther King Holiday

Tue., Jan 22

No Scheduled Laboratory Sessions

Laboratory Is Open 8:00 AM – 4:00 PM
for Practicing Use of the Microscope

Wed., Jan. 23

Thu., Jan. 24

·       Safety Quiz

·       Exercise: Basic Culture Technique: Aseptic Transfer

·       Exercise: Basic Culture Techniques: Streak Plate

·       Lecture Notes: Aseptic Transfer and Streak Plate Techniques

·       LearnSmart Lab Exercise: Lab Safety (closed)

Mon., Jan. 28

Tue., Jan 29

·       Exercise: Bacterial Smears and Simple Staining

·       Lecture Notes: Smears and Simple Staining

·       Skills Test I: Microscopy Proficiency

·       Skills Test II: Streak Plate Proficiency

·       LearnSmart Lab Exercise: Staining (open)

·       LearnSmart Lab Exercise: Microscope Microbiology (closed)

·       LearnSmart Lab Exercise: Aseptic Technique (closed)

·       LearnSmart Lab Exercise: Isolation Methods (closed)

Wed., Jan. 30

Thu., Jan. 31

·       Bacterial Smears and Simple Staining (cont.)

Mon., Feb. 4

Tue., Feb. 5

·       Quiz (smears, simple staining, and Gram stain)

·       Exercise: Differential Staining – The Gram Stain

·       Lecture Notes: Gram Stain

Wed., Feb. 6

Thu., Feb. 7

·       Differential Staining – The Gram Stain (cont.)

·       LearnSmart Lab Exercise: Staining (closed)

Mon., Feb. 11

Tue., Feb. 12

·      Skills Test III: Gram Stain Unknown

·      LearnSmart Lab Exercise: Microbial Growth (open)

·      LearnSmart Lab Exercise: Control of Microbial Growth (open)

The following exercises shall be conducted within groups to be assigned by the laboratory instructor:

·      Exercise: Selective and Differential Media

·      Exercise: Effect of Osmotic Pressure on Microbial Growth

·      Exercise: Effect of Temperature on Microbial Growth

·      Exercise: Effect of pH on Microbial Growth

·      Exercise: The Nitrate Test

·      Lecture Notes: Bacterial Growth Responses

Wed., Feb. 13

Thu., Feb. 14

·       Quiz (current week’s exercises)

·       Selective and Differential Media (cont.)

·       Effect of Osmotic Pressure on Microbial Growth (cont.)

·       Effect of Temperature on Microbial Growth (cont.)

·       Effect of pH on Microbial Growth (cont.)

·       The Nitrate Test (cont.)

Mon., Feb. 18

Tue., Feb. 19

The following exercise shall be conducted within groups to be assigned by the laboratory instructor:

·       Exercise: Determination of Microbial Numbers

·       Lecture Notes: Determination of Microbial Numbers

·       Dilution Problems

Wed., Feb. 20

Thu., Feb. 21

·       Quiz (current week’s exercise)

·       Determination of Microbial Numbers (cont.)

Mon., Feb. 25

Tue., Feb.26

The following exercise shall be conducted within groups to be assigned by the laboratory instructor:

·       Exercise: Antibiotic Susceptibility

·       Lecture Notes: Kirby-Bauer Susceptibility Testing

·       Exercise Addendum: Antibiotic Susceptibility Chart (PDF)

Wed., Feb. 27

Thu., Feb. 28

·       Quiz (current week’s exercise)

·       Antibiotic Susceptibility (cont.)

·      LearnSmart Lab Exercise: Microbial Growth (closed)

·       LearnSmart Lab Exercise: Control of Microbial Growth (closed)

Mon., Mar. 4

Tue., Mar. 5

·      LearnSmart Lab Exercise: Identification of Unknown Bacteria (open)

·      LearnSmart Lab Exercise: Medical Microbiology (open)

The following exercises shall be conducted within groups to be assigned by the laboratory instructor:

Brief Survey of Selected Cocci (Background Reading)

·       Exercise: Hemolysis

·       Exercise: Temperature and Salt Tolerance

·       Exercise: Oxidase Test

·       Exercise: Gelatinase Assay

·       Exercise: DNase Activity

·       Exercise: Coagulase Test

·       Exercise: Catalase Test

·       Exercise: Hydrolysis of Esculin and L-Pyroglutamic
Acid-b-Naphthylamide

·       Exercise: Antibiotic Sensitivity

·       Lecture Notes: A Survey of the Cocci Diagnostics

Wed., Mar. 6

Thu., Mar. 7

·       Quiz (current week’s exercises)

A Survey of Selected Cocci (cont.)

·       Temperature and Salt Tolerance (cont.)

·       Oxidase Test (cont.)

·       Gelatinase Assay (cont.)

·       DNase Activity (cont.)

·       Coagulase Test (cont.)

·       Catalase Test (cont.)

·       Hydrolysis of Esculin and L-Pyroglutamic
Acid-
b-Naphthylamide (cont.)

·       Antibiotic Resistance (cont.)

Mon., Mar. 11
through
Fri., Mar. 15

Laboratory Closed – Spring Break

Mon., Mar. 18

Tue., Mar. 19

The following exercises shall be conducted within groups to be assigned by the laboratory instructor:

A Survey of Selected Bacilli

·       Exercise: Carbohydrate Fermentation

·       Exercise: Sulfide, Indole, Motility Test

·       Exercise: Methyl Red-Voges Proskauer Tests

·       Exercise: Citrate Utilization Test

·       Exercise: Urease Test

·       Lecture Notes: Diagnostic Media, Part I

Wed., Mar. 20

Thu., Mar. 21

·       Quiz (current week’s exercises)

·       LearnSmart Lab Exercise: Identification of Unknown Bacteria (closed)

·       LearnSmart Lab Exercise: Medical Microbiology (closed)

A Survey of Selected Bacilli (cont.)

·      Carbohydrate Fermentation (cont.)

·      Sulfide, Indole, Motility Test (cont.)

·      Methyl Red-Voges Proskauer Tests (cont.)

·      Citrate Utilization Test (cont.)

·      Urease Test (cont.)

Mon., Mar. 25

Tue., Mar. 26

The following exercises shall be conducted within groups to be assigned by the laboratory instructor:

A Survey of Selected Bacilli (cont.)

·       Exercise: Triple Sugar Iron Test

·       Exercise: Decarboxylation Tests

·       Exercise: Indole Test

·       Exercise: Phenylalanine Deamination Test

·       Exercise: Motility Test

·       Lecture Notes: Diagnostic Media, Part II

Wed., Mar. 27

Thu., Mar. 28

·       Quiz (current week’s exercises)

A Survey of Selected Bacilli (cont.)

·       Triple Sugar Iron Test (cont.)

·       Decarboxylation Tests (cont.)

·       Indole Test (cont.)

·       Phenylalanine Deamination Test (cont.)

·       Motility Test (cont.)

Mon., Apr. 1

Tue., Apr. 2

·       Lecture Notes: The Bacterial Unknown Project

·       Skills Test IV: Bacterial Unknowns

Wed., Apr. 3

Thu., Apr. 4

·      Skills Test IV: Bacterial Unknowns (cont.)

Mon., Apr. 8

Tue., Apr. 9

·      Skills Test IV: Bacterial Unknowns (cont.)

Wed., Apr. 10

Thu., Apr. 11

·      Skills Test IV: Bacterial Unknowns (cont.)

Mon., Apr. 15

Tue., Apr. 16

The following exercise shall be conducted within groups to be assigned by the laboratory instructor:

·      Exercise: Isolation and Antibiotic Resistance of Mobile Phone Bacteria

·      Lecture Notes: Mobile Phone Bacteria (to be posted)

Wed., Apr. 17

Thu., Apr. 18

·      Isolation and Antibiotic Resistance of Mobile Phone Bacteria (cont.)

Mon., Apr. 22

Tue., Apr. 23

The following exercise shall be conducted within groups to be assigned by the laboratory instructor:

·       Exercise: Membrane Filter Detection of Coliforms and Enterococci

·       Lecture Notes: Coliform-Enterococcus Isolation

Wed., Apr. 24

Thu., Apr. 25

·       Membrane Filter Detection of Coliforms and Enterococci (cont.)

Mon., Apr. 29

Tue., Apr. 30

Groups will be assigned by the laboratory instructor to conduct one of the following exercises:

·       Exercise: PCR Characterization of Escherichia coli

·       Exercise: PCR Characterization of Enterococcus

·       Lecture Notes: To Be Posted

Wed., May 1

Thu., May 2

·       PCR Characterization of Escherichia coli (cont.)

·       PCR Characterization of Enterococcus (cont.)

Mon., May 6

through

Fri., May 10

Laboratory Closed – Final Exam Week

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© 2018 Chester R. Cooper, Jr.