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© 2022 Chester R. Cooper, Jr. |
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BIOL 3702L: Laboratory Exercises (January
1, 2022) |
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Lecture
Schedule Laboratory
Schedule CONNECT Lecture/Laboratory
Recordings Broken
links? Email Dr.
Cooper |
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This page contains links to downloadable
PDFs of various laboratory exercises and laboratory lecture notes as well as
related materials including videos, instruction manuals, and other suggested
readings. Any links that do not function should be reported by email to Dr.
Cooper. Although this web page contains links to a great
number of laboratory exercises, not all that are listed below will be
assigned during the present semester.
The particular assignments that are assigned
this semester can be found on the Laboratory
Schedule web page.
Students are encouraged to refer to this schedule for specific details
on which exercises shall be assigned during a given week. [ASM recommended competencies
and skills met by a given exercise are noted in
brackets. The ASM recommended
competency/skill #37 applies to all of the following
exercises.] (PDF)
indicates a document requiring Adobe Acrobat Reader Please
report any “broken” links to Dr. Cooper Laboratory Safety (though not an exercise
per se, this is an important topic for students to review) |
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Biosafety in
Microbiological and Biomedical Laboratories (BMBL) 6th Edition
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[37] |
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Standard BIOL
3702L Practices (Open and Read This Web
Page!) This web page has important information
regarding the handling and disposal of materials using this this
laboratory. Students are expected to
abide by these practices. |
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Basic Microbiological Techniques |
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The
Bright-Field Microscope: Its Proper Use and Care
(PDF) Lecture Notes: Bright-Field
Microscopy (PDF) Video: History
of the Microscope Video: How
to Use a Microscope Video: Focusing
a Microscope Video: Focusing
Using Oil Immersion Background Paper: The
Microscope Resource Web Page: MicroscopyU Historical Microscopy: Hooke (1664) Micrographia Instruction Manual: Olympus
Model CH2 Microscope (PDF) Instruction Manual: Olympus
Model CX23 Microscope (PDF) |
[32] |
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[32] |
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Basic
Culture Technique: Aseptic Transfer
(PDF) Lecture Notes: Aseptic
Transfer and Streak Plate Techniques
(PDF) Video: Bunsen
Burner Video: Aseptic
Transfer Handling Two Tubes Simultaneously |
[33, 36] |
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Basic
Culture Technique: Streak Plate
(PDF) Lecture Notes: Aseptic
Transfer and Streak Plate Techniques
(PDF) Video: Performing
a Streak Plate |
[33, 36] |
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[33, 36] |
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Bacterial
Smears and Simple Staining
(PDF) Lecture Notes: Smears
and Simple Staining (PDF) Video: Making
a Bacterial Smear Video: Simple
Stain Technique |
[32, 36] |
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Differential
Staining – The Gram Stain
(PDF) Lecture Notes: Gram
Stain (PDF) Video: The
Gram Stain Historical Paper: Original
Gram Stain Procedure (translated) (PDF) |
[32, 36] |
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[32, 36] |
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Selected Aspects of Microbial Growth Lecture Notes: Bacterial
Growth Responses |
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Differential
and Selective Media (PDF) Hektoen Enteric Agar (PDF) Video: Hemolysis Video: Interpretation
of MSA Growth |
[33,
34, 36] |
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[33,
38] |
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[33,
38] |
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Effect
of pH on Microbial Growth
(PDF) Instructions: Use
of the Spectronic 200E Spectrophotometer Instruction Manual: Spectronic 200E Spectrophotometer
(PDF) |
[35,
36, 38] |
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The
Nitrate Reduction Test
(PDF) Product Insert: Nitrate
Broth Product Insert: Nitrate
Test Reagents Background Paper: History
of the Nitrate Reduction Test Video: Nitrate
Reduction Test |
[33,
36] |
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Aerobic
vs. Anaerobic Culture (PDF) Product Insert: Bio-Bag
Chamber (Type A) |
[35,
36, 38] |
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Pour
Plate Counts (PDF) |
[35,
36, 38] |
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Determination
of Microbial Numbers (PDF) Lecture Notes: Determination
of Microbial Numbers (PDF) Instructions: Use
of the Spectronic 200E Spectrophotometer (PDF) Instruction Manual: Spectronic 200E Spectrophotometer (PDF) Instructions: Use
of the Quebec Colony Counter (PDF) Instruction Manual: Quebec
Colony Country (PDF) Video: Serial
Dilution and Plate Counts Video: How to Use the
Spec 20 Spectrophotometer
Video: Understanding
Serological Pipettes
Video: Using Serological
Pipets
Video: Spread Plate Technique
Video: Using a Micropipette
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[35,
36, 38] |
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Antibiotic
Susceptibility (PDF) Lecture Notes: Kirby-Bauer
Susceptibility Testing
(PDF) Exercise Addendum: Antibiotic
Susceptibility Chart (PDF) |
[36,
38] |
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Exploring the World of Selected Microbes |
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A
Brief Survey of Selected Cocci: Background Discussion
(PDF) Lecture Notes: A
Survey of Cocci Diagnostics
(PDF) |
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Hemolysis
and CAMP Test (PDF) |
[33,
34, 36] |
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Coagulase
Test (PDF) Video: Biology
of Coagulase Test Video: Coagulase
Slide Test |
[33,
34, 36] |
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[33,
34, 36] |
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Oxidase
Test (PDF) |
[33,
34, 36] |
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Gelatinase
Activity (PDF) |
[33,
34, 36] |
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DNase
Activity (PDF) |
[33,
34, 36] |
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Hydrolysis
of Esculin and L-Pyroglutamic Acid-b-Naphthylamide
(PDF) |
[33,
34, 36] |
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Catalase
Test (PDF) Video: Catalase
Test |
[33,
34, 36] |
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Antibiotic
Sensitivity (PDF) |
[33,
34, 36] |
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A
Survey of Selected Bacilli: Background Discussion
(PDF) Lecture Notes: Diagnostic
Media, Part I Lecture Notes: Diagnostic
Media, Part II |
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Carbohydrate
Fermentation (PDF) Product
Insert: Carbohydrate
Fermentation Wee Tab (PDF) (Key Scientific) |
[33,
34, 36] |
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Triple
Sugar Iron Test (PDF) Product Insert: Triple
Sugar Iron Medium (Hardy Diagnostics) Video: Triple
Sugar Iron Test |
[33,
34, 36] |
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Citrate
Utilization Test (PDF) Product Insert: Simmons
Citrate Agar (Hardy Diagnostics) Video: IMViC Tests
[covers several diagnostic tests including the citrate utilization test] |
[33,
34, 36] |
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Methyl
Red-Voges Proskauer Tests
(PDF) Product Insert: Methyl
Red-Voges Proskauer Broth
(Hardy Diagnostics) Product Insert: Methyl
Red Test Reagent (Hardy Diagnostics) Product Insert: Voges-Proskauer
Reagent A (Hardy Diagnostics) Product Insert: Voges-Proskauer
Reagent B (Hardy Diagnostics) Video: IMViC Tests
[covers several diagnostic tests including the methyl red-Voges Proskauer
test] |
[33,
34, 36] |
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Decarboxylation
Tests (PDF) Product Insert: Moellers Decarboxylation Medium (Hardy
Diagnostics) |
[33,
34, 36] |
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Indole
Test (PDF) Product
Insert: Kovacs’
Reagent (Hardy Diagnostics) Video:
IMViC Tests
[covers several diagnostic tests including the indole test] |
[33,
34, 36] |
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Sulfide,
Indole, Motility Test (PDF) Product Insert: SIM
Medium (Hardy Diagnostics) Product Insert: Kovacs’
Reagent (Hardy Diagnostics) |
[33,
34, 36] |
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Phenylalanine
Deamination Test (PDF) Product Insert: Phenylalanine
Deaminase Tabs (Key Scientific) (PDF) |
[33,
34, 36] |
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Urease
Test (PDF) Product Insert: Urease
Test Tablets (Key Scientific) (PDF) Product Insert: Urea/Indole
Wee-Tabs (Key Scientific) (PDF) Product Insert: Kovacs’
Reagent (Hardy Diagnostics) |
[33,
34, 36] |
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Motility
Test (PDF) Product Insert: Motility
Test Medium (Hardy Diagnostics) |
[33,
34, 36] |
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Microbiological Applications in the Real
World |
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Bacterial
Unknowns - Skills Test IV
(PDF) Lecture Notes: The
Bacterial Unknown Project (PDF) Charts of Biochemical and Diagnostic
Features: Selected
Gram-Negative Bacilli Chart
(PDF) Selected
Gram-Positive Bacilli Chart
(PDF) Selected
Cocci Chart (PDF) |
[34,
36, 38] |
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Isolation
and Antibiotic Resistance of Mobile Phone Bacteria
(PDF) Lecture Notes: Mobile Phone Bacteria (to
be posted) |
[36,
38] |
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Membrane
Filter Detection of Coliforms and Enterococci
(PDF) Lecture Notes: Coliform-Enterococcus
Isolation |
[36,
38] |
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PCR
Characterization of Escherichia coli
(PDF) Lecture Notes: To Be Posted Video:
The
Polymerase Chain Reaction (PCR) Video:
Using
a Micropipette Video:
Performing Gel Electrophoresis Video:
SmartDoc 2.0
Imaging System Instructions:
Operation of the Model T100 Thermocycler
(PDF) Instruction
Manual: SmartDoc Imaging System
(PDF) Instruction
Manual: Model
T100 Thermocycler (PDF) |
[34,
36, 38] |
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PCR Characterization of Enterococcus (PDF) Lecture Notes: To Be Posted Video:
The
Polymerase Chain Reaction (PCR) Video:
Using
a Micropipette Video:
Performing Gel Electrophoresis Video:
SmartDoc 2.0
Imaging System Instructions:
Operation of the Model T100 Thermocycler
(PDF) Instruction
Manual: SmartDoc Imaging System
(PDF) Instruction
Manual: Model
T100 Thermocycler (PDF) |
[34,
36, 38] |
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Syllabus Quick Links |
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Laboratory
Videos |
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Laboratory
Exercises |
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Youngstown State University does not discriminate on the basis of race, color, national origin, sex, sexual orientation, gender identity and/or expression, disability, age, religion or veteran/military status in its programs or activities. Please visit the EIT Home Page for contact information for persons designated to handle questions about this policy. It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act. If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical. © 2022 Chester R. Cooper, Jr. |
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