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© 2020 Chester R. Cooper, Jr.

BIOL 3702L Home Page      Laboratory Schedule      Safety Rules

BIOL 3702L: Laboratory Exercises

(updated January 18, 2020)

This page contains links to downloadable PDFs of various laboratory exercises and laboratory lecture notes as well as related materials including videos, instruction manuals, and other suggested readings. Any links that do not function should be reported by email to Dr. Cooper.

The assignment of an exercise to a particular laboratory session can be found on the Laboratory Schedule web page.

[ASM recommended competencies and skills met by a given exercise are noted in brackets.  The ASM recommended competency/skill #37 applies to all of the following exercises.]

(PDF) indicates a document requiring Adobe Acrobat Reader

Please report any “broken” links to Dr. Cooper.

Laboratory Safety (though not an exercise per se, this is an important topic for students to review)

Microbiology Safety Rules

YSU Laboratory Safety Rules

Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition

Video: Microbiology Laboratory Safety

[37]

Standard BIOL 3702L Practices (Open and Read This Web Page!)

This web page has important information regarding the handling and disposal of materials using this this laboratory.  Students are expected to abide by these practices.

Basic Microbiological Techniques

The Bright-Field Microscope: Its Proper Use and Care (PDF)

Lecture Notes: Bright-Field Microscopy (PDF)

Video: History of the Microscope

Video: How to Use a Microscope

Video: Focusing a Microscope

Video: Focusing Using Oil Immersion

Background Paper: The Microscope

Resource Web Page: MicroscopyU

Historical Microscopy: Hooke (1664) Micrographia

Instruction Manual: Olympus Model CH2 Microscope (PDF)

Instruction Manual: Olympus Model CX23 Microscope (PDF)

[32]

Microscopy Proficiency - Skills Test I (PDF)

[32]

Basic Culture Technique: Aseptic Transfer (PDF)

Lecture Notes: Aseptic Transfer and Streak Plate Techniques (PDF)

Video: Bunsen Burner

Video: Aseptic Transfer Handling Two Tubes Simultaneously

Video: Aseptic Transfer Using a Bacti-Cinerator

Video: Aseptic Transfer Using a Bunsen Burner

[33, 36]

Basic Culture Technique: Streak Plate (PDF)

Lecture Notes: Aseptic Transfer and Streak Plate Techniques (PDF)

Video: Performing a Streak Plate

[33, 36]

Streak Plate Proficiency - Skills Test II (PDF)

[33, 36]

Bacterial Smears and Simple Staining (PDF)

Lecture Notes: Smears and Simple Staining (PDF)

Video: Making a Bacterial Smear

Video: Simple Stain Technique

[32, 36]

Differential Staining – The Gram Stain (PDF)

Lecture Notes: Gram Stain (PDF)

Video: The Gram Stain

Historical Paper: Original Gram Stain Procedure (translated) (PDF)

[32, 36]

Gram Stain Unknown - Skills Test III (PDF)

[32, 36]

Selected Aspects of Microbial Growth

Lecture Notes: Bacterial Growth Responses

Differential and Selective Media (PDF)

Hektoen Enteric Agar (PDF)

Video: Hemoloysis

Video: Interpretation of MSA Growth

[33, 34, 36]

Effect of Osmotic Pressure on Microbial Growth (PDF)

[33, 38]

Effect of Temperature on Microbial Growth (PDF)

[33, 38]

Effect of pH on Microbial Growth (PDF)

Instructions: Use of the Spectronic 200E Spectrophotometer

Instruction Manual: Spectronic 200E Spectrophotometer (PDF)

[35, 36, 38]

The Nitrate Reduction Test (PDF)

Product Insert: Nitrate Broth

Product Insert: Nitrate Test Reagents

Background Paper: History of the Nitrate Reduction Test

Video: Nitrate Reduction Test

[33, 36]

Aerobic vs. Anaerobic Culture (PDF)

Product Insert: Bio-Bag Chamber (Type A)

[35, 36, 38]

Pour Plate Counts (PDF)

[35, 36, 38]

Determination of Microbial Numbers (PDF)

Lecture Notes: Determination of Microbial Numbers (PDF)

Instructions: Use of the Spectronic 200E Spectrophotometer

Instruction Manual: Spectronic 200E Spectrophotometer (PDF)

Instructions: Use of the Quebec Colony Counter

Instruction Manual: Quebec Colony Country (PDF)

Video: Serial Dilution and Plate Counts

Video: How to Use the Spec 20 Spectrophotometer

Video: Understanding Serological Pipettes

Video: Using Serological Pipets

Video: Spread Plate Technique

Video: Using a Micropipette

[35, 36, 38]

Antibiotic Susceptibility (PDF)

Lecture Notes: Kirby-Bauer Susceptibility Testing (PDF)

Exercise Addendum: Antibiotic Susceptibility Chart (PDF)

[36, 38]

Exploring the World of Selected Microbes

A Brief Survey of Selected Cocci: Background Discussion (PDF)

Lecture Notes: A Survey of Cocci Diagnostics (PDF)

 

Hemolysis and CAMP Test (PDF)

[33, 34, 36]

Coagulase Test (PDF)

Video: Biology of Coagulase Test

Video: Coagulase Slide Test

[33, 34, 36]

Temperature and Salt Tolerance (PDF)

[33, 34, 36]

Oxidase Test (PDF)

[33, 34, 36]

Gelatinase Activity (PDF)

[33, 34, 36]

DNase Activity (PDF)

[33, 34, 36]

Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide (PDF)

[33, 34, 36]

Catalase Test (PDF)

Video: Catalase Test

[33, 34, 36]

Antibiotic Sensitivity (PDF)

[33, 34, 36]

A Survey of Selected Bacilli: Background Discussion (PDF)

Lecture Notes: Diagnostic Media, Part I

Lecture Notes: Diagnostic Media, Part II

 

Carbohydrate Fermentation (PDF)

Product Insert: Carbohydrate Fermentation Wee Tab (PDF) (Key Scientific)

Video: Carbohydrate Fermentation Test

[33, 34, 36]

Triple Sugar Iron Test (PDF)

Product Insert: Triple Sugar Iron Medium (Hardy Diagnostics)

Video: Triple Sugar Iron Test

[33, 34, 36]

Citrate Utilization Test (PDF)

Product Insert: Simmons Citrate Agar (Hardy Diagnostics)

Video: IMViC Tests [covers several diagnostic tests including the citrate utilization test]

[33, 34, 36]

Methyl Red-Voges Proskauer Tests (PDF)

Product Insert: Methyl Red-Voges Proskauer Broth (Hardy Diagnostics)

Product Insert: Methyl Red Test Reagent (Hardy Diagnostics)

Product Insert: Voges-Proskauer Reagent A (Hardy Diagnostics)

Product Insert: Voges-Proskauer Reagent B (Hardy Diagnostics)

Video: IMViC Tests [covers several diagnostic tests including the methyl red-Voges Proskauer test]

[33, 34, 36]

Decarboxylation Tests (PDF)

Product Insert: Moellers Decarboxylation Medium (Hardy Diagnostics)

[33, 34, 36]

Indole Test (PDF)

Product Insert: Kovacs’ Reagent (Hardy Diagnostics)

Video: IMViC Tests [covers several diagnostic tests including the indole test]

[33, 34, 36]

Sulfide, Indole, Motility Test (PDF)

Product Insert: SIM Medium (Hardy Diagnostics)

Product Insert: Kovacs’ Reagent (Hardy Diagnostics)

[33, 34, 36]

Phenylalanine Deamination Test (PDF)

Product Insert: Phenylalanine Deaminase Tabs (Key Scientific) (PDF)

[33, 34, 36]

Urease Test (PDF)

Product Insert: Urease Test Tablets (Key Scientific) (PDF)

Product Insert: Urea/Indole Wee-Tabs (Key Scientific) (PDF)

Product Insert: Kovacs’ Reagent (Hardy Diagnostics)

[33, 34, 36]

Motility Test (PDF)

Product Insert: Motility Test Medium (Hardy Diagnostics)

[33, 34, 36]

Microbiological Applications in the Real World

Bacterial Unknowns - Skills Test IV (PDF)

Lecture Notes: The Bacterial Unknown Project (PDF)

Charts of Biochemical and Diagnostic Features:

Selected Gram-Negative Bacilli Chart (PDF)

Selected Gram-Positive Bacilli Chart (PDF)

Selected Cocci Chart (PDF)

[34, 36, 38]

Isolation and Antibiotic Resistance of Mobile Phone Bacteria (PDF)

Lecture Notes: Mobile Phone Bacteria (to be posted)

[36, 38]

Membrane Filter Detection of Coliforms and Enterococci (PDF)

Lecture Notes: Coliform-Enterococcus Isolation

[36, 38]

PCR Characterization of Escherichia coli (PDF)

Lecture Notes: To Be Posted

Video: The Polymerase Chain Reaction (PCR)

Video: Using a Micropipette

Video: Performing Gel Electrophoresis

Video: SmartDoc 2.0 Imaging System

Instructions: Operation of the Model T100 Thermocycler (PDF)

Instruction Manual: SmartDoc Imaging System (PDF)

Instruction Manual: Model T100 Thermocycler (PDF)

Background Paper: Omar and Barnard (2014) OhioLink; PubMed

[34, 36, 38]

PCR Characterization of Enterococcus (PDF)

Lecture Notes: To Be Posted

Video: The Polymerase Chain Reaction (PCR)

Video: Using a Micropipette

Video: Performing Gel Electrophoresis

Video: SmartDoc 2.0 Imaging System

Instructions: Operation of the Model T100 Thermocycler (PDF)

Instruction Manual: SmartDoc Imaging System (PDF)

Instruction Manual: Model T100 Thermocycler (PDF)

Background Paper: Nomura et al. (2018) OhioLink; PubMed

[34, 36, 38]

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It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act.  If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical.

© 2020 Chester R. Cooper, Jr.