three bacterial morphology types: coccus, rod, and spirillum  YSU logo  conidiophores of Talaromyces marneffei, a pathogenic fungus

© 2020 Chester R. Cooper, Jr.

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BIOL 3702H Laboratory Schedule

(August 1, 2020)

General Information

A student’s final grade for the course will be based upon the total number of points earned.  Students shall have the opportunity to earn a minimum of 1000 points that are divided between those available via the lecture component of the course and those through the laboratory component,  The pertinent details are provided below.

Weekly Report

The Weekly Research Progress Report is a fillable PDF.  Students need to submit a completed report each Monday no later than 12:00 PM (noon).  The form should be downloaded and the various areas filled using Adobe Acrobat.  The student should then save the file using the following format for the title: “Last Name”-3702H-report-numerical date”.  For example, a weekly report submitted on August 1st by Dr. Cooper should have the title Cooper-3702H-report-080120.  The student should then send this file to Dr. Cooper via email being sure to use the Subject Line of BIOL 3702H Lab Report.

 

Date

Activities

Week of Aug. 17

Introduction to BIOL 3702H

Microbiology Safety Rules

The Bright-Field Microscope: Its Proper Use and Care

Week of Aug. 24

Safety Quiz

Microscopy Proficiency - Skills Test I

Basic Culture Technique: Aseptic Transfer

Basic Culture Technique: Streak Plate

Preparation of media (Protocol To Be Distributed)

Week of Aug. 31

Streak Plate Proficiency - Skills Test II

Differential Staining – The Gram Stain

Overview of semester project (Protocol To Be Distributed)

Week of Sep. 7

Gram Stain Unknown - Skills Test III

Field trip to Mill Creek MetroParks (MCMP) and water sampling; Processing of water sample (Sample 1)

Membrane Filter Isolation of Enterococcus (Protocol To Be Distributed)

Week of Sep. 14

Biochemical characterization of fecal bacteria from Sample 1

Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide

Temperature and Salt Tolerance

Week of Sep. 21

Molecular characterization of fecal bacteria from Sample 1

PCR Characterization of Enterococcus (Protocol To Be Distributed)

Week of Sep. 28

Continue characterization of Sample 1 isolates

Week of Oct. 5

Collect MCMP Sample 2 and characterize enterococci

Week of Oct. 12

Continue characterization of Sample 2 isolates

Week of Oct. 19

Collect MCMP Sample 3 and characterize enterococci

Week of Oct. 26

Continue characterization of Sample 3 isolates

Begin to prepare semester report and public presentation (Instructions To Be Distributed)

Week of Nov. 2

Collect MCMP Sample 4 and characterize enterococci

Continue to prepare semester report and public presentation with submission of preliminary drafts of each

Week of Nov. 9

Continue characterization of Sample 4 isolates

Continue to prepare semester report and public presentation

Week of Nov. 16

Continue laboratory work (if needed)

Continue to prepare semester report and public presentation

Week of Nov. 23

Submit semester report

Week of Nov. 30:

Public presentation of semester results

 

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BIOL 3702/BIOL 3702H
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BIOL 3702 Laboratory
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Honors Laboratory
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It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act.  If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical.

© 2020 Chester R. Cooper, Jr.