Lecture Schedule    Laboratory Schedule   CONNECT    Lecture/Laboratory Recordings

The following BIOL 3702 laboratory schedule is subject to modification as necessary.

In addition, students are strongly encouraged to browse the Laboratory Exercises web page of this syllabus.  It contains additional material and videos that support the activities scheduled below as well enhance a student’s educational experience.

IMPORTANT: Students are solely accountable for adhering to the deadlines noted within this laboratory schedule.  No extensions shall be granted and late submissions shall not be accepted.

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Date

Assignments

Mon., 10 January

and

Tue., 11 January

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Wed., 12 January

and

Thu., 13 January

Introduction to the Microbiology Laboratory [PDF] (Video Lecture)

Assignments:

·      Safety Quiz (Deadline: 1/19 at 8:00 AM)

·      Liability Waiver [Mandatory] (Deadline: 1/21 at 12:00 PM)

·      Virtual Lab Tutorial [Bonus Points] (Deadline: 1/21 at 12:00 PM)

·      Virtual Lab Safety (Hand Washing) (Deadline: 1/21 at 12:00 PM)

·      Virtual Lab Safety (Personal) (Deadline: 1/21 at 12:00 PM)

·      Pre-Lab Quiz #1 (Microscopy) (Deadline: 1/19 at 8:00 AM)

Fri., 14 January

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Sat., 15 January

Weekend – Laboratory is Closed

Sun., 16 January

Mon., 17 January

Holiday – University Closed

Tue., 18 January

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Wed., 19 January

and

Thu., 20 January

Bright-Field Microscopy [PDF] (Video Lecture)

Exercise: The Bright-Field Microscope: Its Proper Use and Care

Assignments:

·      Virtual Lab Brightfield Microscopy (Deadline: 1/28 at 12:00 PM)

·      Virtual Lab Microscopy - Oil Immersion (Deadline: 1/28 at 12:00 PM)

·      Pre-Lab Quiz #2 (Aseptic Technique) (Deadline: 1/24 at 8:00 AM)

Fri., 21 January

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 22 January

Weekend – Laboratory is Closed

Sun., 23 January

Mon., 24 January

through

Thu., 27 January

Aseptic Transfer and Streak Plate Techniques [PDF] (Video Lecture)

Exercise: Basic Culture Technique: Aseptic Transfer

Exercise: Basic Culture Techniques: Streak Plate

Assignments:

·      Skills Test I: Microscopy Proficiency (Deadline: 2/4 at 12:00 PM; submit via Blackboard)

·      Pre-Lab Quiz #3 (Simple Staining) (Deadline: 1/31 at 8:00 AM)

Fri., 28 January

Open Lab: 8:00 AM – 12:30 PM ONLY

Deadline (by 12:00 PM): Microscopy Lab Report (submit via Blackboard)

Sat., 29 January

Weekend – Laboratory is Closed

Sun., 30 January

Mon., 31 January

through

Thu., 3 February

No Labs This Week

Assignments:

·      Virtual Lab Bacterial Smear (Deadline: 2/11 at 12:00 PM)

·      Pre-Lab Quiz #4 (Gram Stain) (Deadline: 2/14 at 8:00 AM)

Fri., 4 February

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 5 February

Weekend – Laboratory is Closed

Sun., 6 February

Mon., 7 February

through

Thu., 10 February

Smears and Simple Staining [PDF] (Video Lecture)

Exercise: Bacterial Smears and Simple Staining

Assignments:

·      Skills Test II: Streak Plate Proficiency (Deadline: 2/18 at 12:00 PM; submit via Blackboard)

·      Virtual Lab Microbial Growth – Oxygen Requirement CANCELLED

·      Pre-Lab Quiz #5 (Bacterial Growth Responses) CANCELLED

Fri., 11 February

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 12 February

Weekend – Laboratory is Closed

Sun., 13 February

Mon., 14 February

through

Thu., 17 February

Gram Stain [PDF] (Video Lecture)

Exercise: Differential Staining – The Gram Stain

Assignments:

·      Pre-Lab Quiz #6 (Microbial Numbers) (Deadline: 2/21 at 8:00 AM)

·      Pre-Lab Quiz #7 (Susceptibility Testing) (Deadline: 2/21 at 8:00 AM)

Fri., 18 February

Open Lab: 8:00 AM – 12:30 PM ONLY

Deadline (by 12:00 PM): Instructor’s choice of laboratory reports – to be announced (submit via Blackboard)

Sat., 19 February

Weekend – Laboratory is Closed

Sun., 20 February

Mon., 21 February

through

Thu., 24 February

Determination of Microbial Numbers [PDF] (Video Lecture)

Exercise: Pour Plate Counts

Kirby-Bauer Susceptibility Testing [PDF] (Video Lecture)

Exercise: Antibiotic Susceptibility (Kirby-Bauer Test)

Exercise Addendum: Antibiotic Susceptibility Chart

Assignment:

·      Skills Test III: Gram Stain Unknown (Deadline: 3/4 at 12:00 PM; submit via Blackboard)

·      Virtual Lab Microbial Control – Antiseptics (Deadline: 3/4 at 12:00 PM)

·      Pre-Lab Quiz #8 (Survey of the Cocci) (Deadline: 2/28 at 8:00 AM)

Fri., 25 February

Open Lab: 8:00 AM – 12:30 PM ONLY

Deadline (by 12:00 PM): Instructor’s choice of laboratory reports – to be announced (submit via Blackboard)

Sat., 26 February

Weekend – Laboratory is Closed

Sun., 27 February

Mon., 28 February

through

Thu., 3 March

A Brief Survey of Selected Cocci: Background Discussion [PDF]

A Survey of Cocci Diagnostics [PDF] (Video Lecture)

Exercise: Hemolysis

Exercise: Oxidase Test

Exercise: Gelatinase Assay

Exercise: DNase Activity

Exercise: Coagulase Test

Exercise: Catalase Test

Exercise: Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide

Exercise: Antibiotic Sensitivity

Assignment:

·      Pre-Lab Quiz #9 (Diagnostic Media I) (Deadline: 3/14 at 8:00 AM)

Fri., 4 March

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 5 March

Weekend – Laboratory is Closed

Sun., 6 March

Mon., 7 March

Spring Break – No Classes

Tue., 8 March

Wed., 9 March

Thu., 10 March

Fri., 11 March

Sat., 12 March

Weekend – Laboratory is Closed

Sun., 13 March

Mon., 14 March

through

Thu., 17 March

A Survey of Selected Bacilli: Background Discussion [PDF]

Survey of the Bacilli: Diagnostic Media I [PDF] (Video Lecture)

Exercise: Carbohydrate Fermentation

Exercise: Indole Test

Exercise: Sulfide, Indole, Motility Test

Exercise: Methyl Red-Voges Proskauer Tests

Exercise: Citrate Utilization Test

Assignments:

·      Virtual Lab Bacterial Unknown [NOTE: Students are assigned the particular exercise corresponding to the last digit of their Y number] (Deadline: 3/25 at 12:00 PM)

·      Pre-Lab Quiz #10 (Diagnostic Media II) (Deadline: 3/21 at 8:00 AM)

Fri., 18 March

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 19 March

Weekend – Laboratory is Closed

Sun., 20 March

Mon., 21 March

through

Thu., 24 March

Survey of the Bacilli: Diagnostic Media II [PDF] (Video Lecture)

Exercise: Urease Test

Exercise: Triple Sugar Iron Test

Exercise: Decarboxylation Tests

Exercise: Phenylalanine Deamination Test

Exercise: Motility Test

Fri., 25 March

Open Lab: 8:00 AM – 12:00 PM ONLY

Deadline (by 12:00 PM): Instructor’s choice of laboratory reports – to be announced (submit via Blackboard)

Sat., 26 March

Weekend – Laboratory is Closed

Sun., 27 March

Mon., 28 March

through

Thu., 31 March

The Bacterial Unknown Project [PDF] (Video Lecture)

Skills Test IV: Bacterial Unknowns (Deadline: 4/15 at 12:00 PM)

Charts of Biochemical and Diagnostic Features:

·      Selected Gram-Negative Bacilli Chart (PDF)

·      Selected Gram-Positive Bacilli Chart (PDF)

·      Selected Cocci Chart (PDF)

Fri., 1 April

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 2 April

Weekend – Laboratory is Closed

Sun., 3 April

Mon., 4 April

through

Thu., 7 April

Bacterial Unknown Project: Skills Test IV (cont.)

Fri., 8 April

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 9 April

Weekend – Laboratory is Closed

Sun., 10 April

Mon., 11 April

through

Thu., 14 April

Bacterial Unknown Project: Skills Test IV (cont.)

Fri., 15 April

Open Lab: 8:00 AM – 12:30 PM ONLY

Deadline (by 12:30 PM): Skills Test IV (submit via Blackboard)

Sat., 16 April

Weekend – Laboratory is Closed

Sun., 17 April

Mon., 18 April

through

Thu., 21 April

Applied Microbiology: Isolation and PCR Characterization of Enterococcus from Wastewater

Fri., 22 April

Open Lab: 8:00 AM – 12:30 PM ONLY

Sat., 23 April

Weekend – Laboratory is Closed

Sun., 24 April

Mon., 25 April

through

Thu., 28 April

Isolation and PCR Characterization of Enterococcus (cont.)

Fri., 29 April

Open Lab: 8:00 AM – 12:30 PM ONLY

Deadline (by 12:00 PM): Enterococcus Report (submit via Blackboard)

Sat., 30 April

Weekend – Laboratory is Closed

Sun., 1 May

Mon., 2 May

through

Fri., 6 May

Finals Week – Laboratory Closed

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