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Laboratory Schedule

Fall 2022 Semester

(August 29, 2022)

LINKS TO IMPORTANT INFORMATION:  Coronavirus Information   Security

Links to Laboratory Syllabus Sections

Laboratory Home Page

Laboratory Schedule

Lecture Home Page

Lecture Schedule

Dr. Cooper’s Contact Information

Ms. Sui’s Contact Information

Laboratory Videos

Laboratory Exercises

Laboratory Lecture Recordings

Laboratory Grading

Laboratory Syllabus Acknowledgment

Liability Waiver

Course Description/
Required Materials

Learning Outcomes

Laboratory Attendance

Assignments and Due Dates

Laboratory Safety Rules

Decorum

Open Lab Periods

ASM Recommended
Competencies/Skills

Important University Policies

Student Support Resources

Sources Used

FERPA

Honors Contracts

Distribution of Course Material

Appreciation

Why This Web Page?

CONNECT Learning Platform

CONNECT Help

Report any broken links/errors to Dr. Cooper (crcooper01@ysu.edu)

NOTE: The Following Schedule is Subject to Change Due to Unforeseen Events

Week 1

Monday, August 29

and

Tuesday, August 30

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Wednesday, August 31

and

Thursday September 1

Introduction to the Microbiology Laboratory [PDF] (Lab Video Lecture)

Check Lab Assignments/Due Dates

Friday, September 2

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Check Lab Assignments/Due Dates

Week 2

Monday, September 5

Labor Day Holiday – University is Closed

Tuesday, September 6

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Last Day to Add BIOL 3702L

Check Lab Assignments/Due Dates

Wednesday, September 7

Thursday, September 8

Bright-Field Microscopy [PDF] (Lab Video Lecture)

Exercise:

·      The Bright-Field Microscope: Its Proper Use and Care

Check Lab Assignments/Due Dates

Friday, September 9

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 3

Monday, September 12

through

Thursday, September 15

Aseptic Transfer and Streak Plate Techniques [PDF] (Lab Video Lecture)

Exercises:

·       Basic Culture Technique: Aseptic Transfer

·       Basic Culture Techniques: Streak Plate

·      Skills Test I: Microscopy Proficiency

Check Lab Assignments/Due Dates

Friday, September 16

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 4

Monday, September 19

through

Thursday, September 22

Smears and Simple Staining [PDF] (Lab Video Lecture)

Exercises:

·       Bacterial Smears and Simple Staining

·      Skills Test II: Streak Plate Proficiency

Check Lab Assignments/Due Dates

Friday, September 23

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 5

Monday, September 26

through

Thursday, September 29

Gram Stain [PDF] (Lab Video Lecture)

Exercise:

·      Differential Staining – The Gram Stain

Check Lab Assignments/Due Dates

Friday, September 30

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 6

Monday, October 3

through

Thursday, October 6

Bacterial Growth Responses [PDF] (Lab Video Lecture)

Exercises:

·       Selective and Differential Media

·       Effect of Osmotic Pressure on Microbial Growth

·      The Nitrate Test

·       Oxygen Requirements/Anaerobic Culture

·       Skills Test III: Gram Stain Unknown

Check Lab Assignments/Due Dates

Friday, October 7

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Last day to apply for fall term graduation

Week 7

Monday, October 10

through

Thursday, October 13

Determination of Microbial Numbers [PDF] (Lab Video Lecture)

Kirby-Bauer Susceptibility Testing [PDF] (Lab Video Lecture)

Exercises:

·      Pour Plate Counts

·      Antibiotic Susceptibility (Kirby-Bauer Test)

Exercise Addendum: Antibiotic Susceptibility Chart

Check Lab Assignments/Due Dates

Friday, October 14

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 8

Monday, October 17

through

Thursday, October 20

A Brief Survey of Selected Cocci: Background Discussion [PDF]

A Survey of Cocci Diagnostics [PDF] (Lab Video Lecture)

Exercises:

·       Hemolysis

·       Oxidase Test

·       Gelatinase Assay

·       DNase Activity

·       Coagulase Test

·       Catalase Test

·       Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide

·      Antibiotic Sensitivity

Check Lab Assignments/Due Dates

Friday, October 21

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 9

Monday, October 24

through

Thursday, October 27

A Survey of Selected Bacilli: Background Discussion [PDF]

Survey of the Bacilli: Diagnostic Media I [PDF] (Lab Video Lecture)

Exercises:

·       Carbohydrate Fermentation

·       Indole Test

·       Sulfide, Indole, Motility Test

·       Methyl Red-Voges Proskauer Tests

·      Citrate Utilization Test

Check Lab Assignments/Due Dates

Friday, October 28

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Sunday, October 30

Last Day to Withdraw with Grade of “W”

Week 10 Assignment

Monday, October 31

and

Tuesday, November 1

Survey of the Bacilli: Diagnostic Media II [PDF] (Lab Video Lecture)

Exercises:

·       Urease Test

·       Triple Sugar Iron Test

·       Decarboxylation Tests

·      Phenylalanine Deamination Test

·       Motility Test

Check Lab Assignments/Due Dates

Wednesday, November 2

and

Thursday, November 3

Bacterial Unknown Project [PDF] (Lab Video Lecture)

Exercise:

·       Skills Test IV: Bacterial Unknown

Check Lab Assignments/Due Dates

Friday, November 4

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 11

Monday, November 7

through

Thursday, November 10

Bacterial Unknown Project (Skills Test IV): cont.

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Friday, November 11

Laboratory Closed – Veterans Day

Week 12

Monday, November 14

through

Thursday, November 17

Bacterial Unknown Project (Skills Test IV): cont.

Check Lab Assignments/Due Dates

Friday, November 18

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 13

Monday, November 21

and

Tuesday, November 22

Bacterial Unknown Project (Skills Test IV): cont.

Check Lab Assignments/Due Dates

NOTE: The laboratory closes at 12:00 PM (Noon) on Tuesday

Wednesday, November 23

through

Friday, November 25

Laboratory Closed – Thanksgiving Holiday

Week 14

Monday, November 28

through

Thursday, December 1

Isolation and Characterization of Enterococcus [PDF] (Lab Video Lecture)

Exercise:

·       Isolation of Enterococcus

Check Lab Assignments/Due Dates

Friday, December 2

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 15

Monday, December 5

through

Thursday, December 8

Exercise:

·       PCR Characterization of Enterococcus

Check Lab Assignments/Due Dates

Friday, December 9

Open Lab: 8:00 AM – Noon ONLY

Check Lab Assignments/Due Dates

Week 16

Monday, December 12
through

Friday, December 16

Laboratory Closed – End of Semester

Check Lab Assignments/Due Dates

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It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act.  If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical.

© 2022 Chester R. Cooper, Jr.