© 2021 Chester R. Cooper, Jr.

The following BIOL 3702 laboratory schedule is subject to modification as necessary.
A PDF of the following schedule is available here (click on underlined link).

Broken links? Email Dr. Cooper

Date

Assignments

Mon., 30 August

and

Tue., 31 August

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Wed., 1 September

and

Thu., 2 September

Introduction to the Microbiology Laboratory [PDF] (Video Lecture)

Assignments (via CONNECT):

·       Safety Quiz

·       Liability Waiver

·       Virtual Lab Tutorial [Bonus Points]

·       Virtual Lab Safety (Hand Washing)

·       Virtual Lab Safety (Personal)

·       Pre-Lab Quiz #1 (Microscopy) (Deadline: prior to the start of your next corresponding lab section)

Fri., 3 September

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Sat., 4 September

and

Sun., 5 September

Weekend – Laboratory is Closed

Mon., 6 September

Holiday – University Closed

Tue., 7 September

NO SCHEDULED LAB ACTIVITIES TODAY

Laboratory is Closed

Deadline (by 8:00 AM): Liability Waiver

Wed., 8 September

and

Thu., 9 September

Bright-Field Microscopy [PDF] (Video Lecture)

Exercise: The Bright-Field Microscope: Its Proper Use and Care

Assignments (via CONNECT):

·      Virtual Lab Brightfield Microscopy

·      Virtual Lab Microscopy - Oil Immersion

Fri., 10 September

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #2 (Aseptic Technique) (Deadline: prior to the start of your next corresponding lab section)

Deadline (by Noon):

·      Safety Quiz

·      Virtual Lab Tutorial

·      Virtual Lab Safety (Hand Washing) Exercise

·      Virtual Lab Safety (Personal) Exercise

Sat., 11 September

and

Sun., 12 September

Weekend – Laboratory is Closed

Mon., 13 September

through

Thu., 16 September

Aseptic Transfer and Streak Plate Techniques [PDF] (Video Lecture)

Exercises:

·      Basic Culture Technique: Aseptic Transfer

·      Basic Culture Techniques: Streak Plate

Skills Test I: Microscopy Proficiency

Fri., 17 September

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #3 (Simple Staining) (Deadline: prior to the start of your next corresponding lab section)

Deadline (by Noon):

·      Microscopy Lab Report (submit via Blackboard)

·      Virtual Lab – Brightfield Microscopy

·      Virtual Lab –Microscopy – Oil Immersion

Sat., 18 September

and

Sun., 19 September

Weekend – Laboratory is Closed

Mon., 20 September

through

Thu., 23 September

Smears and Simple Staining [PDF] (Video Lecture)

Virtual Lab Bacterial Smear

Exercise: Bacterial Smears and Simple Staining

Skills Test II: Streak Plate Proficiency

Fri., 24 September

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #4 (Gram Stain) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Skills Test I (submit via Blackboard)

Sat., 25 September

and

Sun., 26 September

Weekend – Laboratory is Closed

Mon., 27 September

through

Thu., 30 September

Gram Stain [PDF] (Video Lecture)

Exercise: Differential Staining – The Gram Stain

Fri., 1 October

Open Lab: 8:00 AM – Noon ONLY

Assignments (via CONNECT):

·      Virtual Lab Microbial Growth – Oxygen Requirement

·      Pre-Lab Quiz #5 (Bacterial Growth Responses) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon):

·      Skills Test II (submit via Blackboard)

·      Virtual Lab –Bacterial Smear

Sat., 2 October

and

Sun., 3 October

Weekend – Laboratory is Closed

Mon., 4 October

through

Thu., 7 October

Bacterial Growth Responses [PDF] (Video Lecture)

Virtual Lab Microbial Growth – Oxygen Requirement

Exercises:

·      Selective and Differential Media

·      Effect of Osmotic Pressure on Microbial Growth

·      The Nitrate Test

Skills Test III: Gram Stain Unknown

Assignment (via CONNECT):

Fri., 8 October

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #6 (Microbial Numbers) (Deadline: prior to the start of your next corresponding lab section)

Sat., 9 October

and

Sun., 10 October

Weekend – Laboratory is Closed

Mon., 11 October

through

Thu., 14 October

Determination of Microbial Numbers [PDF] (Video Lecture)

Exercise: Pour Plate Counts

Fri., 15 October

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #7 (Susceptibility Testing) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon):

·      Skills Test III (submit via Blackboard)

·      Virtual Lab –Microbial Growth – Oxygen Requirement

·      Instructor’s choice of laboratory reports – to be announced

Sat., 16 October

and

Sun., 17 October

Weekend – Laboratory is Closed

Mon., 18 October

through

Thu., 21 October

Kirby-Bauer Susceptibility Testing [PDF] (Video Lecture)

Exercise: Antibiotic Susceptibility (Kirby-Bauer Test)

Exercise Addendum: Antibiotic Susceptibility Chart

Assignment (via CONNECT): Virtual Lab Microbial Control - Antiseptics

Fri., 22 October

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #8 (Survey of the Cocci) (Deadline: prior to the start of your next corresponding lab section)

Sat., 23 October

and

Sun., 24 October

Weekend – Laboratory is Closed

Mon., 25 October

through

Thu., 28 October

A Brief Survey of Selected Cocci: Background Discussion [PDF]

A Survey of Cocci Diagnostics [PDF] (Video Lecture)

Exercises:

·      Hemolysis

·      Oxidase Test

·      Gelatinase Assay

·      DNase Activity

·      Coagulase Test

·      Catalase Test

·      Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide

·      Antibiotic Sensitivity

Fri., 29 October

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #9 (Diagnostic Media I) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Virtual Lab – Microbial Control - Antiseptics

Sat., 30 October

and

Sun., 31 October

Weekend – Laboratory is Closed

Mon., 1 November

through

Thu., 4 November

A Survey of Selected Bacilli: Background Discussion [PDF]

Survey of the Bacilli: Diagnostic Media I [PDF] (Video Lecture)

Exercises:

·      Carbohydrate Fermentation

·      Indole Test

·      Sulfide, Indole, Motility Test

·      Methyl Red-Voges Proskauer Tests

·      Citrate Utilization Test

Assignment (via CONNECT): Virtual Lab Bacterial Unknown [NOTE: Students are assigned the particular exercise corresponding to the last digit of their Y number]

Fri., 5 November

Open Lab: 8:00 AM – Noon ONLY

Assignment (via CONNECT): Pre-Lab Quiz #10 (Diagnostic Media II) (Deadline: prior to the start of your next corresponding lab section)

Sat., 6 November

and

Sun., 7 November

Weekend – Laboratory is Closed

Mon., 8 November

and

Tue., 9 November

Survey of the Bacilli: Diagnostic Media II [PDF] (Video Lecture)

Exercises:

·      Urease Test

·      Triple Sugar Iron Test

·      Decarboxylation Tests

·      Phenylalanine Deamination Test

·      Motility Test

Wed., 10 November

Open Lab: 8:00 AM – 4:00 PM ONLY

Thu., 11 November

Holiday – University Closed

Fri., 12 November

Open Lab: 8:00 AM – 12:00 PM ONLY

Sat., 13 November

and

Sun., 14 November

Weekend – Laboratory is Closed

Mon., 15 November

through

Thu., 18 November

The Bacterial Unknown Project [PDF] (Skills Test IV: Bacterial Unknowns; Video Lecture)

Charts of Biochemical and Diagnostic Features:

·      Selected Gram-Negative Bacilli Chart (PDF)

·      Selected Gram-Positive Bacilli Chart (PDF)

·      Selected Cocci Chart (PDF)

Fri., 19 November

Open Lab: 8:00 AM – 12:00 PM ONLY

Deadline (Fri. by Noon):

·      Instructor’s choice of laboratory reports – to be announced

·      Virtual Lab – Bacterial Unknown

Sat., 20 November

Open Lab: 9:00 AM – Noon ONLY

Sun., 21 November

Laboratory is Closed

Mon., 22 November

Open Lab: 8:00 AM – 2:00 PM ONLY

Tue., 23 November

Wed., 24 November

No Laboratory Today – University is Open, But Laboratory is Closed

Thu., 25 November

Thanksgiving Holiday – Laboratory is Closed

Fri., 26 November

Thanksgiving Holiday – Laboratory is Closed

Sat., 27 November

and

Sun., 28 November

Weekend – Laboratory is Closed

Mon., 29 November

through

Thu., 2 December

Bacterial Unknown Project (cont.)

Fri., 3 December

Open Lab: 8:00 AM – Noon ONLY

Sat., 4 December

Open Lab: 9:00 AM – Noon ONLY

Sun., 5 December

Laboratory is Closed

Mon., 6 December

through

Thu., 9 December

Bacterial Unknown Project (cont.)

Fri., 10 December

Open Lab: 8:00 AM – Noon ONLY

Deadline (by Noon): Skills Test IV

Sat., 11 December

and

Sun., 12 December

Weekend – Laboratory is Closed

Mon., 13 December

through

Fri., 17 December

Laboratory is Closed for the Semester