© 2021 Chester R. Cooper, Jr.
Lecture Home Page BIOL 3702L Home Page Assignment Deadlines CONNECT Lecture/Laboratory Schedule Lecture/Laboratory Recordings Lecture Schedule (Calendar View)
BIOL 3702 Laboratory Schedule (Calendar View) Spring 2021 Semester (January 18, 2021) This web page contains the laboratory schedule only and is presented from a calendar view. The lecture schedule presented in a calendar view is available on a separate web page. Both of these calendars contain the same information as the master Lecture/Laboratory Schedule. Due to unforeseen events, these schedules and the course components are subject to change. However, to the extent possible, students shall be held harmless should any changes become necessary.
January 2021
Monday	Tuesday	Wednesday	Thursday	Friday
11-14 Introduction to the Microbiology Laboratory [PDF] (Video Lecture) Safety Quiz Liability Waiver Virtual Lab Tutorial [Bonus Points] Virtual Lab Safety (Hand Washing) Virtual Lab Safety (Personal) Pre-Lab Quiz #1 (Microscopy) (Deadline: prior to the start of your next corresponding lab section)				15 Lab Closed to BIOL 3702 Students
18 Legal Holiday – University Closed (No Laboratory Sessions) NOTE: Students in Monday lab sections will not meet this week.	19-21 [Tue., Wed., Thu. lab sections only] Bright-Field Microscopy [PDF] (Video Lecture) Virtual Lab Brightfield Microscopy Virtual Lab Microscopy - Oil Immersion Exercise: The Bright-Field Microscope: Its Proper Use and Care Deadline (Tues. by 8:00 AM): Liability Waiver [Students who fail to submit acknowledgement of the Liability Waiver shall not be permitted to attend any future laboratory sessions until this waiver is successfully completed. In addition, no credit shall be given for work missed.] Deadline (Fri. by Noon): Safety Quiz [Students who fail to pass the Safety Quiz with at least 13 out of 15 possible points shall not be permitted to attend future laboratory sessions until this quiz is successfully completed. In addition, no credit shall be given for work missed.] Deadline (Fri. by Noon): Virtual Lab Tutorial Deadline (Fri. by Noon): Virtual Lab Safety (Hand Washing) Exercise Deadline (Fri. by Noon): Virtual Lab Safety			22 Open Lab Until 12:00 PM
25 [Mon. lab section only] Bright-Field Microscopy [PDF] (Video Lecture Available No Later Than 1/15) Virtual Lab Brightfield Microscopy Virtual Lab Microscopy - Oil Immersion Exercise: The Bright-Field Microscope: Its Proper Use and Care	26-29 [Tue., Wed., and Thu. lab sections shall meet to begin this exercise. Mon. lab sections may begin this exercise as well.] Skills Test I: Microscopy Proficiency Pre-Lab Quiz #2 (Aseptic Technique) (Deadline: prior to the start of your next corresponding lab section) Deadline (Fri. by Noon): Microscopy Lab Report
February 2021
Monday	Tuesday	Wednesday	Thursday	Friday
1-4 Aseptic Transfer and Streak Plate Techniques [PDF] (Video Lecture Available No Later Than 1/29) Exercise: Basic Culture Technique: Aseptic Transfer Exercise: Basic Culture Techniques: Streak Plate [NOTE: Thu. lab sections must return by noon on Friday to check results] Deadline (Fri. by Noon): Skills Test I Deadline (Fri. by Noon): Virtual Lab – Brightfield Microscopy Deadline (Fri. by Noon): Virtual Lab –Microscopy – Oil Immersion Pre-Lab Quiz #3 (Simple Staining) (Deadline: prior to the start of your next corresponding lab section)				5 Open Lab Until 12:00 PM
8-12 Smears and Simple Staining [PDF] (Video Lecture Available No Later Than 2/5) Virtual Lab Bacterial Smear Exercise: Bacterial Smears and Simple Staining Skills Test II: Streak Plate Proficiency Pre-Lab Quiz #4 (Gram Stain) (Deadline: prior to the start of your next corresponding lab section) Deadline (Fri. by Noon): Skills Test II Deadline (Fri. by Noon): Aseptic Technique Lab Reports (both Aseptic Transfer and Streak Plate)				13 Open Lab Until 12:00 PM
15 Gram Stain [PDF] (Video Available No Later Than 2/12) Exercise: Differential Staining – The Gram Stain Deadline (Fri. by Noon): Virtual Lab –Bacterial Smear Pre-Lab Quiz #5 (Bacterial Growth Responses) (Deadline: prior to the start of your next corresponding lab section)	16 No Laboratory Sessions NOTE: Students in Tuesday lab sections will attend the second half of the corresponding Thursday lab section (the last 45 minutes) to make heat-fixed smears to be stained later.	17 Gram Stain [PDF] (Video Available No Later Than 2/12) Exercise: Differential Staining – The Gram Stain	18 Gram Stain [PDF] (Video Available No Later Than 2/12) Exercise: Differential Staining – The Gram Stain NOTE: Students in Thursday lab sections will attend only the first half (45 minutes) of the period to make heat-fixed smears to be stained later.	19 Open Lab Until 12:00 PM
		Deadline (Fri. by Noon): Virtual Lab –Bacterial Smear Pre-Lab Quiz #5 (Bacterial Growth Responses) (Deadline: prior to the start of your next corresponding lab section)
22-25 Bacterial Growth Responses [PDF] (Video Lecture Available No Later Than 2/19) Virtual Lab Microbial Growth – Oxygen Requirement Exercise: Selective and Differential Media Exercise: Effect of Osmotic Pressure on Microbial Growth Exercise: The Nitrate Test Skills Test III: Gram Stain Unknown Deadline (Fri. by Noon): Virtual Lab –Microbial Growth – Oxygen Requirement Pre-Lab Quiz #6 (Microbial Numbers) (Deadline: prior to the start of your next corresponding lab section)				26 Open Lab Until 12:00 PM
March 2021
Monday	Tuesday	Wednesday	Thursday	Friday
1-4 Determination of Microbial Numbers [PDF] (Video Lecture Available No Later Than 2/26) Exercise: Pour Plate Counts Pre-Lab Quiz #7 (Susceptibility Testing) (Deadline: prior to the start of your next corresponding lab section) Deadline (Fri. by Noon): Skills Test III Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced				5 Open Lab Until 12:00 PM
8-9 Kirby-Bauer Susceptibility Testing [PDF] (Video Lecture Available No Later Than 3/5) Virtual Lab Microbial Control - Antiseptics Exercise: Antibiotic Susceptibility (Kirby-Bauer Test) Exercise Addendum: Antibiotic Susceptibility Chart Pre-Lab Quiz #8 (Survey of the Cocci) (Deadline: prior to the start of your next corresponding lab section) NOTE: Students in Wednesday lab sections will attend the second half of the corresponding Monday lab section (the last 45 minutes) to conduct the exercise. Likewise, students in Thursday lab sections will attend the second half of the corresponding Tuesday lab section (the last 45 minutes) to conduct the exercise.		10 No formal lab sessions Open lab 8:00 AM – 4:30 PM [Students in Tuesday/Thursday lab sections should return during this time to complete the Susceptibility Testing exercise.]	11-12 No Laboratory Sessions or Open Lab Times
15-18 A Brief Survey of Selected Cocci: Background Discussion [PDF] A Survey of Cocci Diagnostics [PDF] (Video Lecture Available No Later Than 3/12) Exercise: Hemolysis Exercise: Oxidase Test Exercise: Gelatinase Assay Exercise: DNase Activity Exercise: Coagulase Test Exercise: Catalase Test Exercise: Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide Exercise: Antibiotic Sensitivity Pre-Lab Quiz #9 (Diagnostic Media I) (Deadline: prior to the start of your next corresponding lab section) Deadline (Fri. by Noon): Virtual Lab – Microbial Control - Antiseptics				19 Open Lab Until 12:00 PM
22-25 A Survey of Selected Bacilli: Background Discussion [PDF] Survey of the Bacilli: Diagnostic Media I (Video Lecture Available No Later Than 3/19) Virtual Lab Bacterial Unknown [NOTE: Students are assigned the particular exercise corresponding to the last digit of their Y number] Exercise: Carbohydrate Fermentation Exercise: Indole Test Exercise: Sulfide, Indole, Motility Test Exercise: Methyl Red-Voges Proskauer Tests Exercise: Citrate Utilization Test Pre-Lab Quiz #10 (Diagnostic Media II) (Deadline: prior to the start of your next corresponding lab section) Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced				26 Open Lab Until 12:00 PM
29-April 1 Survey of the Bacilli: Diagnostic Media II (Video Lecture Available No Later Than 3/26) Exercise: Urease Test Exercise: Triple Sugar Iron Test Exercise: Decarboxylation Tests Exercise: Indole Test Exercise: Phenylalanine Deamination Test Exercise: Motility Test				April 2 Open Lab Until 12:00 PM
April 2021
Monday	Tuesday	Wednesday	Thursday	Friday
5-6 The Bacterial Unknown Project [PDF] (Skills Test IV: Bacterial Unknowns; Video Lecture Available No Later Than 4/2) Charts of Biochemical and Diagnostic Features: · Selected Gram-Negative Bacilli Chart (PDF) · Selected Gram-Positive Bacilli Chart (PDF) · Selected Cocci Chart (PDF) NOTE: Students in Wednesday lab sections will attend the second half of the corresponding Monday lab section (the last 45 minutes) to conduct the exercise. Students in Thursday’s lab section will attend the second half of the corresponding Tuesday lab section (the last 45 minutes) to conduct the exercise. Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced Deadline (Fri. by Noon): Virtual Lab Bacterial Unknown		7 No Laboratory Sessions	8 NO FORMAL LAB MEETING See Monday/Tuesday instructions Open Lab 8:00 AM – 12:30 PM and 4:00 PM – 6:00 PM	9 Open Lab Until 12:00 PM
Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.
12-15 Bacterial Unknown (cont.) Open Lab – See Posted Schedule				16 Open Lab Until 12:00 PM
Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.
19-22 Bacterial Unknown (cont.) Open Lab – See Posted Schedule				23 Open Lab Until 12:00 PM
Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.
26-29 Bacterial Unknown (cont.) Open Lab – See Posted Schedule Deadline (Fri. by Noon): Skills Test IV				16 Open Lab Until 12:00 PM
Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.
May 2021
Monday	Tuesday	Wednesday	Thursday	Friday
3-7 LABS CLOSED FOR THE SEMESTER

Coronavirus Safety Measures	Lecture Home Page	BIOL 3702L Home Page
Lecture/Laboratory Schedule	Assignment Deadlines	Lecture/Laboratory Recordings
Course Description	Learning Outcomes	Course Materials
Dr. Cooper’s Contact Information/Office Hours	Syllabus Changes	Attendance
Syllabus Acknowledgement	Liability Waiver	Grading
Open Lab Schedule	Decorum	Security
Lecture Videos	Laboratory Videos [links to important videos]	ASM Recommended Competencies and Skills (PDF)
Other Important Information	Special Accommodations	Laboratory Exercises [links to PDFs/other materials]
Email Communications	Appreciation	Laboratory Safety Rules
Sources Used	Standard Lab Practices
Around the Micro Lab (under development)	Distribution of Course Material
Instructor’s Manual (under development)	Why This Web Page?
Youngstown State University does not discriminate on the basis of race, color, national origin, sex, sexual orientation, gender identity and/or expression, disability, age, religion or veteran/military status in its programs or activities. Please visit the EIT Home Page for contact information for persons designated to handle questions about this policy. It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act. If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical. © 2021 Chester R. Cooper, Jr.