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© 2021 Chester R. Cooper, Jr.

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Lecture Schedule (Calendar View)

BIOL 3702 Laboratory Schedule (Calendar View)

Spring 2021 Semester

(January 18, 2021)

This web page contains the laboratory schedule only and is presented from a calendar view.  The lecture schedule presented in a calendar view is available on a separate web page.  Both of these calendars contain the same information as the master Lecture/Laboratory Schedule.  Due to unforeseen events, these schedules and the course components are subject to change.  However, to the extent possible, students shall be held harmless should any changes become necessary.

January 2021

Monday

Tuesday

Wednesday

Thursday

Friday

11-14

Introduction to the Microbiology Laboratory [PDF] (Video Lecture)

Safety Quiz

Liability Waiver

Virtual Lab Tutorial [Bonus Points]

Virtual Lab Safety (Hand Washing)

Virtual Lab Safety (Personal)

Pre-Lab Quiz #1 (Microscopy) (Deadline: prior to the start of your next corresponding lab section)

 

15

Lab Closed to BIOL 3702 Students

 

18

Legal Holiday – University Closed
(No Laboratory Sessions)

NOTE: Students in Monday lab sections will not meet this week.   

 

19-21

[Tue., Wed., Thu. lab sections only]

Bright-Field Microscopy [PDF] (Video Lecture)

Virtual Lab Brightfield Microscopy

Virtual Lab Microscopy - Oil Immersion

Exercise: The Bright-Field Microscope: Its Proper Use and Care

Deadline (Tues. by 8:00 AM): Liability Waiver [Students who fail to submit acknowledgement of the Liability Waiver shall not be permitted to attend any future laboratory sessions until this waiver is successfully completed.  In addition, no credit shall be given for work missed.]

Deadline (Fri. by Noon): Safety Quiz [Students who fail to pass the Safety Quiz with at least 13 out of 15 possible points shall not be permitted to attend future laboratory sessions until this quiz is successfully completed.  In addition, no credit shall be given for work missed.]

Deadline (Fri. by Noon): Virtual Lab Tutorial

Deadline (Fri. by Noon): Virtual Lab Safety (Hand Washing) Exercise

Deadline (Fri. by Noon): Virtual Lab Safety

 

22

Open Lab Until
12:00 PM

 

25

[Mon. lab section only]

Bright-Field Microscopy [PDF] (Video Lecture Available No Later Than 1/15)

Virtual Lab Brightfield Microscopy

Virtual Lab Microscopy - Oil Immersion

Exercise: The Bright-Field Microscope: Its Proper Use and Care

 

26-29

[Tue., Wed., and Thu. lab sections shall meet to begin this exercise.  Mon. lab sections may begin this exercise as well.]

Skills Test I: Microscopy Proficiency 

Pre-Lab Quiz #2 (Aseptic Technique) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Microscopy Lab Report

 

February 2021

Monday

Tuesday

Wednesday

Thursday

Friday

1-4

Aseptic Transfer and Streak Plate Techniques [PDF] (Video Lecture Available No Later Than 1/29)

Exercise: Basic Culture Technique: Aseptic Transfer

Exercise: Basic Culture Techniques: Streak Plate

[NOTE: Thu. lab sections must return by noon on Friday to check results]

Deadline (Fri. by Noon): Skills Test I

Deadline (Fri. by Noon): Virtual Lab – Brightfield Microscopy

Deadline (Fri. by Noon): Virtual Lab –Microscopy – Oil Immersion

Pre-Lab Quiz #3 (Simple Staining) (Deadline: prior to the start of your next corresponding lab section)

 

5

Open Lab Until
12:00 PM

 

8-12

Smears and Simple Staining [PDF] (Video Lecture Available No Later Than 2/5)

Virtual Lab Bacterial Smear

Exercise: Bacterial Smears and Simple Staining

Skills Test II: Streak Plate Proficiency

Pre-Lab Quiz #4 (Gram Stain) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Skills Test II

Deadline (Fri. by Noon): Aseptic Technique Lab Reports (both Aseptic Transfer and Streak Plate)

 

13

Open Lab Until 12:00 PM

 

15

Gram Stain [PDF] (Video Available No Later Than 2/12)

Exercise: Differential Staining – The Gram Stain

Deadline (Fri. by Noon): Virtual Lab –Bacterial Smear

Pre-Lab Quiz #5 (Bacterial Growth Responses) (Deadline: prior to the start of your next corresponding lab section)

 

16

No Laboratory Sessions

NOTE: Students in Tuesday lab sections will attend the second half of the corresponding Thursday lab section (the last 45 minutes) to make heat-fixed smears to be stained later. 

 

17

Gram Stain [PDF] (Video Available No Later Than 2/12)

Exercise: Differential Staining – The Gram Stain

 

18

Gram Stain [PDF] (Video Available No Later Than 2/12)

Exercise: Differential Staining – The Gram Stain

NOTE: Students in Thursday lab sections will attend only the first half (45 minutes) of the period to make heat-fixed smears to be stained later. 

 

19

Open Lab Until
12:00 PM

 

Deadline (Fri. by Noon): Virtual Lab –Bacterial Smear

Pre-Lab Quiz #5 (Bacterial Growth Responses) (Deadline: prior to the start of your next corresponding lab section)

 

22-25

Bacterial Growth Responses [PDF] (Video Lecture Available No Later Than 2/19)

Virtual Lab Microbial Growth – Oxygen Requirement

Exercise: Selective and Differential Media

Exercise: Effect of Osmotic Pressure on Microbial Growth

Exercise: The Nitrate Test

Skills Test III: Gram Stain Unknown

Deadline (Fri. by Noon): Virtual Lab –Microbial Growth – Oxygen Requirement

Pre-Lab Quiz #6 (Microbial Numbers) (Deadline: prior to the start of your next corresponding lab section)

 

26

Open Lab Until
12:00 PM

 

March 2021

Monday

Tuesday

Wednesday

Thursday

Friday

1-4

Determination of Microbial Numbers [PDF] (Video Lecture Available No Later Than 2/26)

Exercise: Pour Plate Counts

Pre-Lab Quiz #7 (Susceptibility Testing) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Skills Test III

Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced

 

5

Open Lab Until
12:00 PM

 

8-9

Kirby-Bauer Susceptibility Testing [PDF] (Video Lecture Available No Later Than 3/5)

Virtual Lab Microbial Control - Antiseptics

Exercise: Antibiotic Susceptibility (Kirby-Bauer Test)

Exercise Addendum: Antibiotic Susceptibility Chart

Pre-Lab Quiz #8 (Survey of the Cocci) (Deadline: prior to the start of your next corresponding lab section)

NOTE: Students in Wednesday lab sections will attend the second half of the corresponding Monday lab section (the last 45 minutes) to conduct the exercise.  Likewise, students in Thursday lab sections will attend the second half of the corresponding Tuesday lab section (the last 45 minutes) to conduct the exercise.

 

10

No formal lab sessions

 Open lab
8:00 AM – 4:30 PM

[Students in Tuesday/Thursday lab sections should return during this time to complete the Susceptibility Testing exercise.]

 

11-12

No Laboratory Sessions or Open Lab Times

 

15-18

A Brief Survey of Selected Cocci: Background Discussion [PDF]

A Survey of Cocci Diagnostics [PDF] (Video Lecture Available No Later Than 3/12)

Exercise: Hemolysis

Exercise: Oxidase Test

Exercise: Gelatinase Assay

Exercise: DNase Activity

Exercise: Coagulase Test

Exercise: Catalase Test

Exercise: Hydrolysis of Esculin and L-Pyroglutamic Acid-b-Naphthylamide

Exercise: Antibiotic Sensitivity

Pre-Lab Quiz #9 (Diagnostic Media I) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Virtual Lab – Microbial Control - Antiseptics

 

19

Open Lab Until
12:00 PM

 

22-25

A Survey of Selected Bacilli: Background Discussion [PDF]

Survey of the Bacilli: Diagnostic Media I (Video Lecture Available No Later Than 3/19)

Virtual Lab Bacterial Unknown [NOTE: Students are assigned the particular exercise corresponding to the last digit of their Y number]

Exercise: Carbohydrate Fermentation

Exercise: Indole Test

Exercise: Sulfide, Indole, Motility Test

Exercise: Methyl Red-Voges Proskauer Tests

Exercise: Citrate Utilization Test

Pre-Lab Quiz #10 (Diagnostic Media II) (Deadline: prior to the start of your next corresponding lab section)

Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced

 

26

Open Lab Until
12:00 PM

 

29-April 1

Survey of the Bacilli: Diagnostic Media II (Video Lecture Available No Later Than 3/26)

Exercise: Urease Test

Exercise: Triple Sugar Iron Test

Exercise: Decarboxylation Tests

Exercise: Indole Test

Exercise: Phenylalanine Deamination Test

Exercise: Motility Test

 

April 2

Open Lab Until
12:00 PM

 

April 2021

Monday

Tuesday

Wednesday

Thursday

Friday

5-6

The Bacterial Unknown Project [PDF] (Skills Test IV: Bacterial Unknowns; Video Lecture Available No Later Than 4/2)

Charts of Biochemical and Diagnostic Features:

·       Selected Gram-Negative Bacilli Chart (PDF)

·       Selected Gram-Positive Bacilli Chart (PDF)

·       Selected Cocci Chart (PDF)

NOTE: Students in Wednesday lab sections will attend the second half of the corresponding Monday lab section (the last 45 minutes) to conduct the exercise.

Students in Thursday’s lab section will attend the second half of the corresponding Tuesday lab section (the last 45 minutes) to conduct the exercise.

Deadline (Fri. by Noon): Instructor’s choice of laboratory reports – to be announced

Deadline (Fri. by Noon): Virtual Lab Bacterial Unknown

 

7

No Laboratory Sessions

 

8

NO FORMAL LAB MEETING

See Monday/Tuesday instructions

Open Lab

8:00 AM – 12:30 PM
and 4:00 PM – 6:00 PM

 

9

Open Lab Until
12:00 PM

 

Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.

12-15

Bacterial Unknown (cont.)

Open Lab – See Posted Schedule

 

16

Open Lab Until
12:00 PM

 

Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.

19-22

Bacterial Unknown (cont.)

Open Lab – See Posted Schedule

 

23

Open Lab Until
12:00 PM

 

Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.

26-29

Bacterial Unknown (cont.)

Open Lab – See Posted Schedule

Deadline (Fri. by Noon): Skills Test IV

 

16

Open Lab Until
12:00 PM

 

Note: Due to coronavirus restrictions, only 12 students permitted in the laboratory at a time.

May 2021

Monday

Tuesday

Wednesday

Thursday

Friday

3-7

LABS CLOSED FOR THE SEMESTER

 

 

 

 

Coronavirus Safety
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Why This Web Page?

Youngstown State University does not discriminate on the basis of race, color, national origin, sex, sexual orientation, gender identity and/or expression, disability, age, religion or veteran/military status in its programs or activities. Please visit the EIT Home Page for contact information for persons designated to handle questions about this policy.

It is possible that portions of this web page are not wholly in accord with the American with Disabilities Act.  If this is the case, a student currently enrolled in this course should contact Dr. Cooper (crcooper01@ysu.edu; 330.941.1361) and compliant material shall be provided as soon as practical.

© 2021 Chester R. Cooper, Jr.